Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Vigna angularis | 5618 | - |
extracellular | extracellular or apoplastic space of epicotyls | Vigna angularis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE | Vigna angularis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
donor xyloglucan + xyloglucan acceptor | Vigna angularis | reconnecting enzyme for xyloglucans, involved in the interweaving or reconstruction of cell wall matrix, which is responsible for chemical creepage that leads to morphological changes in the cell wall, responsible for cell wall loosening and integration of cell wall architecture | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna angularis | - |
Ohwi and Ohashi, cv. Takara | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | with mannosyl residues | Vigna angularis |
Purification (Comment) | Organism |
---|---|
87fold, from apoplastic space of epicotyls | Vigna angularis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epicotyl | - |
Vigna angularis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Vigna angularis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
donor xyloglucan + acceptor xyloglucan | acceptor specificity | Vigna angularis | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | enzyme requires a basic xyloglucan structure, i.e. a beta-1,4-glucosyl backbone with xylosyl side chains, for both acceptor and donor activity, higher activity when xyloglucans with higher MW are used as donor substrates, xyloglucans smaller than 10 kDa are no donor substrates, pyridylamino heptasaccharide, xyloglucan oligomers or polymers are good acceptors | Vigna angularis | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | highly specific for xyloglucan as the glycosyl donor | Vigna angularis | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | xyloglucan from Tamarindus indica | Vigna angularis | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | xyloglucan from Vigna angularis | Vigna angularis | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | xyloglucan from Tropaeolum majus seed | Vigna angularis | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | donor specificity | Vigna angularis | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | enzyme transfers a large segment of a xyloglucan molecule to another one generating chimeric polymers | Vigna angularis | ? | - |
? | |
donor xyloglucan + acceptor xyloglucan | galactosyl or fucosyl side chains are not required for acceptor activity | Vigna angularis | ? | - |
? | |
donor xyloglucan + Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta1-PA | pyridylamino heptasaccharide is a good substrate | Vigna angularis | ? | - |
? | |
donor xyloglucan + xyloglucan acceptor | reconnecting enzyme for xyloglucans, involved in the interweaving or reconstruction of cell wall matrix, which is responsible for chemical creepage that leads to morphological changes in the cell wall, responsible for cell wall loosening and integration of cell wall architecture | Vigna angularis | ? | - |
? | |
additional information | no glycosidase or glycanase activity, no donors: xyloglucans smaller than 10 kDa, carboxymethylcellulose, Avena glucan, maize xylan, Rhodymenia xylan, no acceptors: pyridylamino cellohexaose, pyridylamino laminarihexaose | Vigna angularis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE | Vigna angularis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Vigna angularis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
- |
Vigna angularis |